PubMed Holland LM et al., PLoS pathogens, 2014 Sep;10(9):e1004365Holland LM et al., PLoS pathogens, 2014 Sep;10(9):e1004365
KMP1
Direct
EMSA
Expression of GST-KlYap8 was induced by incubating E. coli BL21(DE3)pLysS cells with 1 mM IPTG (isopropyl BETA-D-thiogalactoside) for 4 h at 30.C in LB medium in the presence of 100 microg/ml ampicillin and 34 microg/ml chloramphenicol.