K.lactis - N.C. Yeastract

Regulation Reference(s)

The N.C. Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
KLLA0E00265p	KLLA0_E00243g	PubMed Veide Vilg J et al., Biochim Biophys Acta, 2014 Nov;1839(11):1295-306	Direct	EMSA	N/A	KMP1	Expression of GST-KlYap8 was induced by incubating E. coli BL21(DE3)pLysS cells with 1 mM IPTG (isopropyl BETA-D-thiogalactoside) for 4 h at 30.C in LB medium in the presence of 100 microg/ml ampicillin and 34 microg/ml chloramphenicol.
			Indirect	Microarray analysis - WT vs TF mutant	Positive	KMP1	Exponentially growing wild type and Klyap8DELTA cells (OD600 of approx. 0.6) in YPD medium were either untreated or exposed to stress (0.1 mM sodium arsenite, NaAsO2) for 30 minutes
			Indirect	Microarray analysis - WT vs TF mutant	Positive	KMP1	Exponentially growing wild type and Klyap8DELTA cells (OD600 of approx. 0.6) in YPD medium were either untreated or exposed to stress (0.25 mM tert-butyl hydroperoxide, t-BOOH) for 30 minutes