PubMed
Ruth C et al., Microb Cell Fact, 2014 Sep 3;13(0):120Ruth C et al., Microb Cell Fact, 2014 Sep 3;13(0):120
Indirect
Microarray analysis - WT vs TF mutant
Positive
CBS7435
Cultures, containing 2 mL M2 medium, were inoculated to an optical density OD600 of 4.0. The synthetic medium M2 contained per liter: 22.0 g Citric acid monohydrate, 3.15 g (NH4)2PO4, 0.49 g MgSO4.7H2O, 0.80 g KCl, 0.0268 g CaCl2.2H2O, 1.47 mL of PTM1 trace metals and 4 mg Biotin; pH was set to 5 with KOH (solid).