PubMed
Chen C et al., Cell host & microbe, 2011 Aug 18;10(2):118-35Chen C et al., Cell host & microbe, 2011 Aug 18;10(2):118-35
Indirect
Microarray analysis - WT vs TF Deletion
Positive
SN250
Ion/metal/phosphate/sulfur/vitamin availability
Cells were inoculated into YPD (OD600=10⁻⁴), grown overnight, then diluted to OD600 0.01 in low iron media, incubated at 30°C for 5–6 hours, and harvested at OD600 0.5–0.6. Transcription factor gene deletion.
0.81636383
PubMed
Junier A et al., mSphere, 2022 Feb 23;7(1):e0077921Junier A et al., mSphere, 2022 Feb 23;7(1):e0077921
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
pcz1
Ion/metal/phosphate/sulfur/vitamin availability
Grown overnight in a complete minimal medium minus uridine (CM-U) at 25°C. The cultures were treated with 4 μM of calcium ionophore A23187. After 30 min of treatment, cells were harvested and frozen in RNAlater. Transcription factor gene deletion.
0.352608857823217
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
pcz1
Stress
Grown overnight in a complete minimal medium minus uridine (CM-U) at 25°C. The cultures were treated with a vehicle control (100% ethanol). After 30 min of treatment, cells were harvested and frozen in RNAlater.Transcription factor gene deletion.
0.185295779998971
PubMed
Xiong L et al., mSphere, 2024 Jul 30;9(7):e0037224Xiong L et al., mSphere, 2024 Jul 30;9(7):e0037224
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SC5314
Ion/metal/phosphate/sulfur/vitamin availability
BPS: Cells were pre-cultured in liquid YPD (2% dextrose, 2% Bacto peptone, 1% yeast extract) rotating at 75 rpm at 30°C overnight. Cells were inoculated into 25 mL of pre-warmed (37°C) YPD + 500 µM BPS (bathophenanthrolinedisulfonate; iron chelator) at an initial OD600 of 0.2. Cultures were grown for 4 h with vigorous shaking (225 rpm) at 37°C, then harvested by vacuum filtration and immediately frozen at -80°C.
