PubMed
Teunissen JHM et al., BMC genomics, 2017 Sep 6;18(1):701Teunissen JHM et al., BMC genomics, 2017 Sep 6;18(1):701
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
SC5314
Unstressed log-phase growth (control)
Grown in YPD media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Transcription factor gene deletion.
-0.272990681740646
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SC5314
Stress
Grown in YPD media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Butyric acid was added to freshly relaunched overnight cultures, diluted down to an optical density at 600 nm of 0.1. Transcription factor gene deletion.
0.368723827067921
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SC5314
Stress
Grown in YPD media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Acetic acid was added to freshly relaunched overnight cultures, diluted down to an optical density at 600 nm of 0.1. Transcription factor gene deletion.
0.267596846381842
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SC5314
Stress
Grown in YPM media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Acetic acid were added to freshly relaunched overnight cultures, diluted down to an optical density at 600 nm of 0.1. Transcription factor gene deletion.