PubMed
Teunissen JHM et al., BMC genomics, 2017 Sep 6;18(1):701Teunissen JHM et al., BMC genomics, 2017 Sep 6;18(1):701
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SC5314
Unstressed log-phase growth (control)
Grown in YPD media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Transcription factor gene deletion.
0.273518906287565
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SC5314
Stress
Grown in YPD media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Butyric acid was added to freshly relaunched overnight cultures, diluted down to an optical density at 600 nm of 0.1. Transcription factor gene deletion.
1.18552804459534
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
SC5314
Stress
Grown in YPD media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Acetic acid was added to freshly relaunched overnight cultures, diluted down to an optical density at 600 nm of 0.1. Transcription factor gene deletion.
-0.890370454547236
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SC5314
Stress
Grown in YPM media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Acetic acid were added to freshly relaunched overnight cultures, diluted down to an optical density at 600 nm of 0.1. Transcription factor gene deletion.
0.577577908329473
PubMed
Lagree K et al., PLoS genetics, 2020 Jan;16(1):e1008582Lagree K et al., PLoS genetics, 2020 Jan;16(1):e1008582
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
CW542
Unstressed log-phase growth (control)
Grown in a YPD medium for 4 h shaking at 225 rpm at 37°C. Transcription factor gene deletion.