PubMed
Teunissen JHM et al., BMC genomics, 2017 Sep 6;18(1):701Teunissen JHM et al., BMC genomics, 2017 Sep 6;18(1):701
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SC5314
Unstressed log-phase growth (control)
Grown in YPD media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Transcription factor gene deletion.
0.243969764193491
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SC5314
Stress
Grown in YPD media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Acetic acid was added to freshly relaunched overnight cultures, diluted down to an optical density at 600 nm of 0.1. Transcription factor gene deletion.
0.428796898550449
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
SC5314
Carbon source quality/availability
Grown in YPM media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Transcription factor gene deletion.
-0.451221910221195
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SC5314
Stress
Grown in YPM media at 37 °C in a shaking incubator at 150 rpm. Stock cultures were preserved in 35% glycerol and maintained at −80 °C. Acetic acid were added to freshly relaunched overnight cultures, diluted down to an optical density at 600 nm of 0.1. Transcription factor gene deletion.
