PubMed
Finkel JS et al., PLoS pathogens, 2012 Feb;8(2):e1002525Finkel JS et al., PLoS pathogens, 2012 Feb;8(2):e1002525
Indirect
Microarray analysis - WT vs TF Deletion
Positive
DAY185
Cell cycle/morphology
Grown from overnight YPD cultures and inoculated into 50 ml Spider medium at OD 0.2 for 8 hours at 37°C with 220-rpm agitation. Transcription factor gene deletion.
0.96790056
PubMed
Fanning S et al., Eukaryotic cell, 2012 Jul;11(7):896-904Fanning S et al., Eukaryotic cell, 2012 Jul;11(7):896-904
Indirect
Microarray analysis - WT vs TF Deletion
Positive
DAY185
Cell cycle/morphology
Cells were grown in Spider medium (1% nutrient broth, 1% d-mannitol, 0.2% K2HPO4). Transcription factor gene deletion.
1.269171
PubMed
Huang MY et al., PLoS pathogens, 2019 May;15(5):e1007787Huang MY et al., PLoS pathogens, 2019 May;15(5):e1007787
Indirect
NanoString wt vs TF mutant
Negative
P87
Cell cycle/morphology
Hyphal growth (RPMI + 10% serum at 37°C for 4 hours)
N/A
PubMed
Meza-Davalos T et al., Molecular microbiology, 2025 Oct;124(4):327-341Meza-Davalos T et al., Molecular microbiology, 2025 Oct;124(4):327-341
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
SN250
Cell cycle/morphology
Grown in Lee’s medium (pH 4.5) at 30 °C for 18 h, washed, then ~2 × 10⁶ cells were transferred to inducing media (Milli-Q H₂O with 10% FBS) and incubated at 37 °C for 1 h at 200 rpm to induce filamentation. Transcription factor gene deletion.
