PubMed
Porman AM et al., PLoS genetics, 2013 Mar;9(3):e1003369Porman AM et al., PLoS genetics, 2013 Mar;9(3):e1003369
Indirect
Microarray analysis - TF Over-expression vs WT
Positive
CAY3378
Cell cycle/morphology
Cultures were inoculated in 3 ml of Spider medium then incubated at 25°C overnight. 2 ODs of cells were spun down and resuspended in 1 ml of Lee's + Glucose medium and transferred to the well of a 12-well polystyrene plate. Transcription factor gene overexpression.
N/A
PubMed
Anderson MZ et al., PLoS genetics, 2016 Oct;12(10):e1006353Anderson MZ et al., PLoS genetics, 2016 Oct;12(10):e1006353
Direct
RT-PCR - wt vs mutant promoter sequence
Negative
MYA-3404
Cell cycle/morphology
White cells
N/A
PubMed
Zheng Q et al., Molecular microbiology, 2017 Dec;106(6):999-1017Zheng Q et al., Molecular microbiology, 2017 Dec;106(6):999-1017