PubMed
Murad AM et al., Molecular microbiology, 2001 Nov;42(4):981-93Murad AM et al., Molecular microbiology, 2001 Nov;42(4):981-93
Indirect
Microarray analysis - wt vs TF mutant
Positive
JM43
Aerobiosis; cells grown in SSG-TEA (10 g/L galactose, 0.1% Tween 80, 20 mg/L ergosterol, 350 ppm silicone antifoam) or YPGalactose medium
N/A
PubMed
Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
N/A
PubMed
Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488
Indirect
Microarray analysis - WT vs TF Deletion
Negative
BY4741
Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Transcription factor gene deletion.
