PubMed
Bester MC et al., Current genetics, 2006 Jun;49(6):375-83Bester MC et al., Current genetics, 2006 Jun;49(6):375-83
Indirect
Northern blot - wt vs TF overexpression
Positive
S288c
cells grown to stationary phase in synthetic medium
N/A
PubMed
Bester MC et al., G3 (Bethesda, Md.), 2012 Jan;2(1):131-41Bester MC et al., G3 (Bethesda, Md.), 2012 Jan;2(1):131-41
Direct
RT-PCR - wt vs mutant promoter sequence
Positive
S288c
Minimal medium; mid-log phase
N/A
Indirect
Microarray analysis - TF Over-expression vs WT
Positive
S288c
Yeast grown in 5 mL (test tubes on rotary wheel) synthetic dropout media (YNB) with 2% glucose (SCD media) at 30 °C to early stationary phase (OD600 ̴ 1-2). Transcription factor gene overexpression.
6.43123008
PubMed
Judeh T et al., Nucleic acids research, 2013 Feb 1;41(3):1425-37Judeh T et al., Nucleic acids research, 2013 Feb 1;41(3):1425-37