PubMed
Tachibana C et al., Molecular and cellular biology, 2005 Mar;25(6):2138-46Tachibana C et al., Molecular and cellular biology, 2005 Mar;25(6):2138-46
Direct
ChIP
N/A
S288c
Carbon source quality/availability
Growth in glucose derepression conditions (0.05% glucose)
N/A
PubMed
Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Negative
N/A
Carbon source quality/availability
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
N/A
PubMed
Wierman MB et al., Molecular and cellular biology, 2017 Jul 1;37(13):Wierman MB et al., Molecular and cellular biology, 2017 Jul 1;37(13):
Indirect
RT-PCR - wt vs TF mutant
Positive
BY4741
Carbon source quality/availability
SC medium -18h
N/A
Indirect
RT-PCR - wt vs TF mutant
Positive
BY4741
Carbon source quality/availability
SC medium with 0.5% glucose -18h
N/A
PubMed
Horkai D et al., PLoS biology, 2023 Aug;21(8):e3002245Horkai D et al., PLoS biology, 2023 Aug;21(8):e3002245
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
MEP
Carbon source quality/availability
YPD,48h: Cells were grown in YP + 2% glucose (YPD) at 30°C, 200 rpm and mother cells were harvested 48h after estradiol addition (added at 2h post-inoculation).