PubMed
Laskar S et al., Molecular and cellular biology, 2015 Jan;35(2):437-50Laskar S et al., Molecular and cellular biology, 2015 Jan;35(2):437-50
Indirect
Western blot - wt vs TF mutant
Positive
S288c
Cells carrying the empty vector vs the Hsp82 overexpression plasmid (SLY77), with anti-Sir2 antibodies, SC medium
N/A
PubMed
Read T et al., PLoS genetics, 2016 Jan;12(1):e1005746Read T et al., PLoS genetics, 2016 Jan;12(1):e1005746
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
L6441
Cells were grown in 100 ml of CSD (Chelex-treated synthetic defined medium) containing <100 nM of zinc. Cells were inoculated from overnight cultures into CSD at an initial OD600 = 0.01 and grown to mid-log phase (OD600 ≈ 0.5) before RNA isolation.
0.465400674191727
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
L6441
Grown to mid-log phase in standard YPD media. Transcription factor gene deletion.