PubMed
Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804
Direct
Chip-on-chip
N/A
S288c
Unstressed log-phase growth (control)
YPD medium; mid-log phase
N/A
PubMed
Vizoso-Vázquez A et al., Applied microbiology and biotechnology, 2012 Apr;94(1):173-84Vizoso-Vázquez A et al., Applied microbiology and biotechnology, 2012 Apr;94(1):173-84
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4741
Unstressed log-phase growth (control)
Grown in SC medium with defined amino acid supplements and 2% glucose at 30 °C, in Erlenmeyer flasks filled to 40% volume and shaken at 250 rpm. Transcription factor gene deletion.
0.62798138
PubMed
Krakowiak J et al., eLife, 2018 Feb 2;7():Krakowiak J et al., eLife, 2018 Feb 2;7():
Indirect
Microarray analysis - WT vs TF Deletion
Positive
W303a
Unstressed log-phase growth (control)
Grown overnight in complete SD medium with specific amino acid supplements and 2% glucose, then diluted to OD600 0.4 in fresh medium and cultured at 30 °C with shaking until mid-log phase (OD600 0.6). Transcription factor gene deletion.
