S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Pho4p	YDR481C	PubMed Barbarić S et al., Nucleic acids research, 1992 Mar 11;20(5):1031-8	Direct	EMSA	N/A	S288c	In vitro	in vitro	N/A
		PubMed Hayashi N et al., Molecular and cellular biology, 1991 Feb;11(2):785-94	Direct	EMSA	N/A	S288c	In vitro	in vitro	N/A
		PubMed Munsterkötter M et al., The Journal of biological chemistry, 2000 Jul 28;275(30):22678-85	Direct	lacZ - wt vs mutant promoter sequence	Positive	S288c	Ion/metal/phosphate/sulfur/vitamin availability	Phosphate depletion	N/A
		PubMed Ogawa N et al., Molecular biology of the cell, 2000 Dec;11(12):4309-21	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	Exponential phase cells cultivated in YPD	N/A
		PubMed Huang D et al., Molecular and cellular biology, 2002 Jul;22(14):5076-88	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY263	Unstressed log-phase growth (control)	Cells grown to log phase in YPD medium	N/A
		PubMed Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF mutant	Positive	N/A	Unstressed log-phase growth (control)	Cells grown in synthetic medium supplemented with 2% dextrose	N/A
		PubMed Nishizawa M et al., PLoS biology, 2008 Dec 23;6(12):2817-30	Direct	ChIP	N/A	S288c	Unstressed log-phase growth (control)	YNB medium without phosphate + 2mM (high Pi)	N/A
		PubMed Aow JS et al., Nucleic acids research, 2013 May;41(9):4877-87	Direct	ChIP	N/A	S288c	Ion/metal/phosphate/sulfur/vitamin availability	low phosphate	N/A
		PubMed Tsang F et al., The Journal of biological chemistry, 2015 May 15;290(20):12753-64	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	Control	N/A
		PubMed He BZ et al., eLife, 2017 May 9;6():He BZ et al., eLife, 2017 May 9;6():	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	K699	Unstressed log-phase growth (control)	100 ml of cell culture grown to OD600nm (600 nm) of 1.5.	1.94842213691641
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	K699	Ion/metal/phosphate/sulfur/vitamin availability	Grown in phosphate-free SC medium (Phosphate starvation) at 30°C with shaking. All transcriptional profiling experiments were done in the pho80∆ background. Transcription factor gene deletion.	1.94842213691641
		PubMed Gurvich Y et al., PLoS biology, 2017 Dec;15(12):e2002039	Indirect	RNA-seq - wt vs TF mutant	Positive	BY4741	Ion/metal/phosphate/sulfur/vitamin availability	BY4741; SC medium; Phosphate(i) starvation condition; 30.C; mid-log phase	N/A
			Indirect	RNA-seq - wt vs TF mutant	Positive	BY4741 PHO4SA12346 allele	Ion/metal/phosphate/sulfur/vitamin availability	BY4741 PHO4SA12346 allele ; SC medium; Low P(i) concentration; 30.C; mid-log phase	N/A
			Indirect	RNA-seq - wt vs TF mutant	Positive	BY4741 PHO84-constitutive	Ion/metal/phosphate/sulfur/vitamin availability	BY4741 PHO84c; SC medium; yeast cell recovery into 20mM P(i) from low P(i); 30.C; 2h growth	N/A
		PubMed van Heusden GPH, Genomics, 2023 Sep;115(5):110672	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	BY4741	Ion/metal/phosphate/sulfur/vitamin availability	Grown overnight at 30 °C in phosphate-free YNB medium supplemented with 7.2 mM potassium phosphate (pH 5.8), 20 mg/L histidine, leucine, methionine, and uracil, and 50 mM KCl, then used to inoculate 50 mL cultures at OD620 = 0.1 and grown at 30 °C, 180 rpm to OD620 = 0.5. Transcription factor gene deletion.	0.317352845034005