PubMed
Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():
Indirect
RNA-seq analysis - WT vs TF Mutant
N/A
FY4
Cell cycle/morphology
Early first acidic phase (day 4): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 4, corresponding to the early first acidic phase.
0.829484748
Indirect
RNA-seq analysis - WT vs TF Mutant
N/A
FY4
Nitrogen source quality/availability
Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-1 (W321). Transcription factor gene mutation.
0.829484748
PubMed
Sato A et al., Bioscience, biotechnology, and biochemistry, 2021 Feb 24;85(3):587-599Sato A et al., Bioscience, biotechnology, and biochemistry, 2021 Feb 24;85(3):587-599
Indirect
RT-PCR - wt vs. TF double mutant
Positive
X2180-1B
Nitrogen source quality/availability
Nitrogen starved cells (Cells were grown in YPD medium to an early logarithmic phase. For nitrogen starvation, cells were washed once and further cultured for 6 h in SD-N ((0.17% yeast nitrogen base without amino acids and ammonium sulfate, and 2% glucose)).
