PubMed
Rutherford JC et al., Proceedings of the National Academy of Sciences of the United States of America, 2001 Dec 4;98(25):14322-7Rutherford JC et al., Proceedings of the National Academy of Sciences of the United States of America, 2001 Dec 4;98(25):14322-7
Direct
DNA footprinting
Positive
S288c
Exponential phase cells cultivated in CM growth medium
N/A
PubMed
Courel M et al., Molecular and cellular biology, 2005 Aug;25(15):6760-71Courel M et al., Molecular and cellular biology, 2005 Aug;25(15):6760-71
Indirect
Microarray analysis - wt vs TF mutant
Negative
Y14438
Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C.
N/A
PubMed
Dubacq C et al., Molecular genetics and genomics : MGG, 2006 Feb;275(2):114-24Dubacq C et al., Molecular genetics and genomics : MGG, 2006 Feb;275(2):114-24
Indirect
Microarray analysis - wt vs TF mutant
Positive
S288c
YPD medium + 200mM hydroxyurea
N/A
PubMed
Berthelet S et al., Genetics, 2010 Jul;185(3):1111-28Berthelet S et al., Genetics, 2010 Jul;185(3):1111-28
Indirect
Microarray analysis - WT vs TF Deletion
Negative
YKB779
Grown in YPD at 30°C to OD600 of 0.4. Transcription factor gene deletion.