PubMed
Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804
Direct
Chip-on-chip
N/A
S288c
YPD medium; mid-log phase
N/A
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104
Direct
Chip-on-chip
N/A
S288c
YPD medium; mid-log phase
N/A
PubMed
Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488
Indirect
Microarray analysis - WT vs TF Deletion
Negative
BY4741
Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Transcription factor gene deletion.
-0.638696
Indirect
Microarray analysis - WT vs TF Deletion
Negative
BY4741
Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Yeast cells grown to OD600 0.6 and treated with 0.1 mM H2O2 for 45 minutes. Transcription factor gene deletion.