S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition	Log2FC
Rox1p	YGL021W	PubMed Becerra M et al., Molecular microbiology, 2002 Feb;43(3):545-55	Indirect	Microarray analysis - wt vs TF mutant	Negative	aGH1	Cells grown in complete medium with 0.5% glucose in aerobic conditions	N/A
		PubMed Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Negative	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h	N/A
		PubMed Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488	Indirect	Microarray analysis - WT vs TF Deletion	Negative	BY4741	Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Transcription factor gene deletion.	-0.82068
			Indirect	Microarray analysis - WT vs TF Deletion	Negative	BY4741	Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Yeast cells grown to OD600 0.6 and treated with 0.1 mM H2O2 for 45 minutes. Transcription factor gene deletion.	-0.845491
		PubMed van der Horst SC et al., Nature communications, 2025 Feb 6;16(1):1416	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	W303	0 min: RNA-seq performed in YPAD medium. Overnight cultures were diluted in fresh YPAD and grown for 3 h. Cells were then synchronized in G1 by α-factor treatment for 2 h, washed once in YPAD, and released into YPAD containing 200 mM hydroxyurea (HU). Samples were collected at t=0min: immediately after α-factor synchronization (pre-HU release)	-0.154843
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	W303	60 min: RNA-seq performed in YPAD medium. Overnight cultures were diluted in fresh YPAD and grown for 3 h. Cells were then synchronized in G1 by α-factor treatment for 2 h, washed once in YPAD, and released into YPAD containing 200 mM hydroxyurea (HU). Samples were collected at t=60min: 60 min after release into HU (replication stress condition)	-0.273419