PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104
Direct
Chip-on-chip
N/A
S288c
YPD medium; mid-log phase
N/A
PubMed
Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488
Indirect
Microarray analysis - WT vs TF Deletion
Negative
BY4741
Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Transcription factor gene deletion.
-0.726686
Indirect
Microarray analysis - WT vs TF Deletion
Negative
BY4741
Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Yeast cells grown to OD600 0.6 and treated with 0.1 mM H2O2 for 45 minutes. Transcription factor gene deletion.
-0.540105
PubMed
van der Horst SC et al., Nature communications, 2025 Feb 6;16(1):1416van der Horst SC et al., Nature communications, 2025 Feb 6;16(1):1416
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
W303
0 min: RNA-seq performed in YPAD medium. Overnight cultures were diluted in fresh YPAD and grown for 3 h. Cells were then synchronized in G1 by α-factor treatment for 2 h, washed once in YPAD, and released into YPAD containing 200 mM hydroxyurea (HU). Samples were collected at t=0min: immediately after α-factor synchronization (pre-HU release)
