S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Rox1p	YKL008C	PubMed Murad AM et al., Molecular microbiology, 2001 Nov;42(4):981-93	Indirect	Microarray analysis - wt vs TF mutant	Negative	JM43 / RZ53-6	Carbon source quality/availability	Aerobiosis; cells grown in SSG-TEA (10 g/L galactose, 0.1% Tween 80, 20 mg/L ergosterol, 350 ppm silicone antifoam) or YPGalactose medium	N/A
		PubMed Ter Linde JJ et al., Yeast (Chichester, England), 2002 Jul;19(10):825-40	Indirect	Microarray analysis - wt vs TF mutant	Negative	CEN.PK113-7D	Carbon source quality/availability	Steady-state chemostat cultures aerobically grown on mineral mediium containing 7.5 g/L glucose (growth-limiting nutrient)	N/A
		PubMed Kolaczkowski M et al., Eukaryotic cell, 2004 Aug;3(4):880-92	Direct	DNA footprinting	N/A	SEY6210	In vitro	in vitro	N/A
		PubMed Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488	Indirect	Microarray analysis - WT vs TF Deletion	Negative	BY4741	Unstressed log-phase growth (control)	Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Transcription factor gene deletion.	-2.229363
			Indirect	Microarray analysis - WT vs TF Deletion	Negative	BY4741	Stress	Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Yeast cells grown to OD600 0.6 and treated with 0.1 mM H2O2 for 45 minutes. Transcription factor gene deletion.	-1.991325
		PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Negative	BY4741; S288C; R1158	Unstressed log-phase growth (control)	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	-1.1446930997917
		PubMed Horkai D et al., PLoS biology, 2023 Aug;21(8):e3002245	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	MEP	Carbon source quality/availability	YPD,48h: Cells were grown in YP + 2% glucose (YPD) at 30°C, 200 rpm and mother cells were harvested 48h after estradiol addition (added at 2h post-inoculation).	-1.101626
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	MEP	Unstressed log-phase growth (control)	YPD,log: Cells were grown in YP + 2% glucose (YPD) at 30°C, 200 rpm and harvested at log phase as a young control.	-1.291494
		PubMed van der Horst SC et al., Nature communications, 2025 Feb 6;16(1):1416	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	W303	Cell cycle/morphology	0 min: RNA-seq performed in YPAD medium. Overnight cultures were diluted in fresh YPAD and grown for 3 h. Cells were then synchronized in G1 by α-factor treatment for 2 h, washed once in YPAD, and released into YPAD containing 200 mM hydroxyurea (HU). Samples were collected at t=0min: immediately after α-factor synchronization (pre-HU release)	-1.68208
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	W303	Cell cycle/morphology	60 min: RNA-seq performed in YPAD medium. Overnight cultures were diluted in fresh YPAD and grown for 3 h. Cells were then synchronized in G1 by α-factor treatment for 2 h, washed once in YPAD, and released into YPAD containing 200 mM hydroxyurea (HU). Samples were collected at t=60min: 60 min after release into HU (replication stress condition)	-1.432143