PubMed
Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Negative
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
N/A
PubMed
Badis G et al., Molecular cell, 2008 Dec 26;32(6):878-87Badis G et al., Molecular cell, 2008 Dec 26;32(6):878-87
Direct
ChIP
N/A
S288c
N/A
N/A
PubMed
Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4741; S288C; R1158
Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.
0.44299353642191
PubMed
Platt JM et al., Genes & development, 2013 Jun 15;27(12):1406-20Platt JM et al., Genes & development, 2013 Jun 15;27(12):1406-20
Indirect
RT-PCR - wt vs TF mutant
Positive
S288c
Senescence
N/A
PubMed
Byun JY et al., Microbial cell factories, 2025 Feb 24;24(1):47Byun JY et al., Microbial cell factories, 2025 Feb 24;24(1):47
Indirect
RNA-seq - wt vs TF overexpression
Positive
CEN.PK2-1D
YPD medium (15h) (diauxic shift)
1.18513833550234
Indirect
RNA-seq analysis - TF Over-expression vs WT
Positive
cen.pk-2-1C
RNA extraction at 15h. Medium: YPD (1% yeast extract, 2% peptone, 2% glucose) or synthetic complete (SC) medium supplemented as required for auxotrophic markers. Temperature: 30°C, aerobic shaking. Growth phase: exponential or stationary phase.