S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gat1p	YLR142W	PubMed Steber CM et al., Proceedings of the National Academy of Sciences of the United States of America, 1995 Dec 19;92(26):12490-4	Indirect	Northern blot - wt vs TF mutant	Positive	TCY1	Nitrogen source quality/availability	Wickerham's minimal medium + GABA	N/A
		PubMed Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	30 min 50 nM treatment with rapamycin in YPD	N/A
		PubMed Saxena D et al., Eukaryotic cell, 2003 Jun;2(3):552-9	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	Glutamate - yeast cells were grown in minimal medium containing glutamate (Glt, 0.1%).	N/A
		PubMed Leverentz MK et al., The Journal of biological chemistry, 2009 Sep 4;284(36):24115-22	Direct	ChIP	N/A	S288c	N/A	N/A	N/A
		PubMed Zhao X et al., Scientific reports, 2016 Feb 22;6():21603	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	YNB medium, with glutamine or proline	N/A
		PubMed Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():	Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Early first acidic phase (day 4): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 4, corresponding to the early first acidic phase.	1.371752523
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-1 (W321). Transcription factor gene mutation.	1.371752523