S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gis1p	YNR073C	PubMed Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Carbon source quality/availability	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h	N/A
		PubMed Zhang N et al., The Journal of biological chemistry, 2010 Feb 26;285(9):6465-76	Indirect	Microarray analysis - TF Over-expression vs WT	Positive	CML376	Unstressed log-phase growth (control)	Cells were grown to mid-exponential phase under doxycycline repression (A₆₀₀ ≈ 0.4), then washed and resuspended in medium with or without doxycycline for 3 h. Transcription factor tetO-GIS1 overexpression.	0.61065269
			Indirect	Microarray analysis - TF Over-expression vs WT	Positive	CML376	Unstressed log-phase growth (control)	Cells were grown to mid-exponential phase under doxycycline repression (A₆₀₀ ≈ 0.4), then washed and resuspended in medium with or without doxycycline for 6 h. Transcription factor tetO-GIS1 overexpression.	1.35559519
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	Unstressed log-phase growth (control)	YPD medium, 25.C	N/A
		PubMed Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	BY4741	Unstressed log-phase growth (control)	Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.	-0.853747605942238