PubMed
Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488Kelley R et al., PLoS genetics, 2009 May;5(5):e1000488
Indirect
Microarray analysis - WT vs TF Deletion
Negative
BY4741
Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Transcription factor gene deletion.
-1.004523
Indirect
Microarray analysis - WT vs TF Deletion
Negative
BY4741
Single colony overnight growth in 10 mL of YPD at 30°C resuspended in 100 mL of YPD media at an OD600 of 0.1 and placed in an orbital shaker at 30°C. Yeast cells grown to OD600 0.6 and treated with 0.1 mM H2O2 for 45 minutes. Transcription factor gene deletion.
-1.286831
PubMed
Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4741; S288C; R1158
Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.