Grown overnight at 30 °C in phosphate-free YNB medium supplemented with 7.2 mM potassium phosphate (pH 5.8), 20 mg/L histidine, leucine, methionine, and uracil, and 50 mM KCl, then used to inoculate 50 mL cultures at OD620 = 0.1 and grown at 30 °C, 180 rpm to OD620 = 0.5. Transcription factor gene deletion.