PubMed
Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804
Direct
Chip-on-chip
N/A
S288c
YPD medium; mid-log phase
N/A
PubMed
Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
S90
Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase.
0.440202149060286
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
S90
Grown in CDM35 at 30 ÂșC and harvested at OD600 of 1. Transcription factor gene deletion.