PubMed
Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Carbon source quality/availability
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
N/A
PubMed
Höfken T, Biochemical and biophysical research communications, 2017 Dec 2;493(4):1485-1490Höfken T, Biochemical and biophysical research communications, 2017 Dec 2;493(4):1485-1490
Indirect
lacZ - wt vs TF mutant
Positive
THY612
Unstressed log-phase growth (control)
wt and DELTAecm22DELTAupc2 cells carrying a PRM4-lacZ plasmid were grown in synthetic complete (SC) medium
N/A
Indirect
lacZ - wt vs TF mutant
Positive
THY612
Cell cycle/morphology
wt and DELTAecm22DELTAupc2 cells carrying a PRM4-lacZ plasmid were incubated with 10 mg/ml alpha-factor for 150 min to test response to pheromone