PubMed
Krakowiak J et al., eLife, 2018 Feb 2;7():Krakowiak J et al., eLife, 2018 Feb 2;7():
Indirect
Microarray analysis - WT vs TF Deletion
Negative
W303a
Grown overnight in complete SD medium with specific amino acid supplements and 2% glucose, then diluted to OD600 0.4 in fresh medium and cultured at 30 °C with shaking until mid-log phase (OD600 0.6). Transcription factor gene deletion.
-0.55290333
Indirect
Microarray analysis - WT vs TF Deletion
Negative
W303a
Grown overnight in complete SD medium with specific amino acid supplements and 2% glucose, then diluted to OD600 0.4 in fresh medium and cultured at 30 °C with shaking until mid-log phase (OD600 0.6). Cisplatin treatment (600 μM in DMSO), incubated for 4 hours at 30 °C in the dark with agitation. Transcription factor gene deletion.